from November 13-19, sFn was held. it's basically a convention for all the nerds in the neuro field to go to. i didn't attend it cause i've nothing to present :( maybe next year. anyway, my lab is completely empty except for a couple undergraduate that hardly show up. so i was doing western blotting, which is some very nice way of separating protein according to their weight. it starts with some homogenization, which basically means i'm squashing some brain cell in buffer solution. follow by immunoprecipitation (not going thought the steps), a difmup test and finally western blotting.
now the everything went fine until i started the western blotting. Basically i needed to transfer my samples into ten lanes that is also filled with another buffer. now the problem was that i didn't write down what's the concentration of the buffer suppose to be, and being new, i didn't know everything is suppose to be 1X not 4X or 5X. i just assume that different experiment will use different concentration... so i load 5X of running buffer.
the results were fantastic... in not a good way... i had 6 sample and 2 molecular marker... so i had to load them in one by one... the funny thing was by the time i load the 3rd sample, i felt something was wrong... my sample didn't want to settle down... like they won't go down... i thought maybe i need to give it some time... so i continue to load the rest of the sample and molecular marker... when i was done the result was amazing... the first two sample i load was GONE... POOF INTO THE AIR... somebody stole my sample? well, not exactly... i look at the third sample and guess what i saw?
the sample was CLIMBING OUT OF THE LANE. it's a freaking sample and it's climbing out... oh yeah, i'm loosing sample by the minute... so yeah, i'm slightly screwed... well maybe completely screwed cause i can't continue without my sample... it took me a while to figure out my mistake... so i related it to my coworker at the sFn...
guess what they do? they set up a facebook group just for me... oh yeah, and it's a secret facebook group... OHHH... didn't know you could do that...
so since, i'm telling you all right now, "it's not a secret anymore!"
*applause* *applause* *applause*
oh yeah, on a side note. if you think you never get enough static shock... come to the US during winter and you will be in for a surprise. i get a daily regiment of static shock every time i get back to my room... if i'm REALLY REALLY LUCKY, i'll get one on my way to class :P
what an intersting place to live in.
another side note. this is my thanksgiving dinner provided by the school one week in advance
ok, so it does look weird in the picture. but who cares. i have turkey and bread on the right. mash potato, corn and crumbs with gravy on the left... what a nice meal... and don't forget, a cup of chocolate milk :P
2 comments:
the someone in particular is not particularly pissed but at least you could link back to me right? right?? lol
i feel my arm being twisted from behind :P
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